| Strain, strain background (Mus musculus) |
C56Bl/6J |
JAX |
JAX 000664, RRID:IMSR_JAX:000664
|
|
| Strain, strain background (Mus musculus) |
Il10-/-
|
JAX |
JAX 002251, RRID:IMSR_JAX:002251
|
|
| Strain, strain background (Mus musculus) |
Il10fl/fl |
PMID:15534372, PMID:28783045
|
from Dr. Werner Muller via Dr. Asma Nusrat |
|
| Strain, strain background (Mus musculus) |
Foxp3creYFP |
JAX |
JAX 016959, RRID:IMSR_JAX:016959
|
|
| Strain, strain background (Mus musculus) |
Il10fl/fl x Foxp3creYFP (IL-10 cKO) |
This paper |
JAX 016959, RRID:IMSR_JAX:016959 and from Dr. Werner Muller via Dr. Asma Nusrat |
We crossed IL-10fl/fl mice obtained from Dr. Asma Nusrat with commercially available FoxP3-creYFP mice. To obtain, contact the Cobb lab. |
| Strain, strain background (Mus musculus) |
BALB/c Foxp3GFP
|
JAX |
JAX 006769, RRID:IMSR_JAX:006769
|
|
| Strain, strain background (Mus musculus) |
Foxp3RFP |
JAX |
JAX 008374, RRID:IMSR_JAX:008374
|
|
| Strain, strain background (Mus musculus) |
Il10GFP |
JAX |
JAX 008379, RRID:IMSR_JAX:008379
|
|
| Strain, strain background (Mus musculus) |
Foxp3RFP x Il10GFP (Xmas) |
This paper |
JAX 008374, RRID:IMSR_JAX:008374 and JAX 008379, RRID:IMSR_JAX:008379
|
We crossed two commercially available mouse lines. To obtain, contact the Cobb lab. |
| Antibody |
αCD4-magnetic beads (rat, monoclonal) |
Miltenyi Biotec |
130-049-201, RRID:AB_2722753
|
(1:50–1:100), (50 µL) |
| Antibody |
αCD3 (Armenian hamster, monoclonal) |
eBioscience |
16-0031-85, RRID: AB_468848
|
(1:400), (50 µL) |
| Antibody |
αCD28 (Syrian hamster, monoclonal) |
eBioscience |
16-0281-82, RRID: AB_468921
|
(1:400), (50 µL) |
| Antibody |
CD4-PE (rat, monoclonal) |
eBioscience |
12-0042-82, RRID: AB_465510
|
(1:250–1:500), (50 µL) |
| Antibody |
CD4-AF647 (rat, monoclonal) |
Biolegend |
100530, RRID: AB_389325
|
(1:250–1:500), (50 µL) |
| Antibody |
CD4-BV421 (rat, monoclonal) |
BD |
740007, RRID: AB_2739779
|
(1:250–1:500), (50 µL) |
| Antibody |
F4/80-BV711 (rat, monoclonal) |
BD |
565612, RRID: AB_2734769
|
(1:250–1:500), (50 µL) |
| Antibody |
CD25-PECy7 (rat, monoclonal) |
BD |
561780, RRID: AB_10893596
|
(1:250–1:500), (50 µL) |
| Antibody |
CD25-PE (rat, monoclonal) |
eBioscience |
12-0251-82, RRID: AB_465607
|
(1:250–1:500), (50 µL) |
| Antibody |
CD124-BB700 (rat, monoclonal) |
BD |
742172, RRID: AB_2871410
|
(1:250–1:500), (50 µL) |
| Antibody |
CD122-APC (rat, monoclonal) |
BD |
564924, RRID: AB_2739009
|
(1:250–1:500), (50 µL) |
| Antibody |
CD132-BV421 (rat, monoclonal) |
BD |
740039, RRID: AB_2739809
|
(1:250–1:500), (50 µL) |
| Antibody |
CD213a1-Biotin (rabbit, polyclonal) |
MyBioSource |
MBS2001753 |
(1:250–1:500), (50 µL) |
| Antibody |
pSTAT3-AF647 (mouse, monoclonal) |
BD |
557815, RRID: AB_647144
|
(1:100–1:200), (25 µL) |
| Antibody |
pSTAT5-AF647 (mouse, monoclonal) |
BD |
612599, RRID: AB_399882
|
(1:100–1:200), (25 µL) |
| Antibody |
pSTAT6-AF647 (mouse, monoclonal) |
BD |
558242, RRID: AB_647145
|
(1:100–1:200), (25 µL) |
| Antibody |
IL-10R biotin (rat, monoclonal) |
Biolegend |
112704, RRID: AB_313517
|
(1:250–1:500), (50 µL) |
| Antibody |
SA-APCefluor780 |
eBioscience |
47-4317-82, RRID: AB_10366688
|
(1:250–1:500), (50 µL) |
| Antibody |
SA-BV605 |
BD |
563260, RRID: AB_2869476
|
(1:250–1:500), (50 µL) |
| Peptide, recombinant protein |
Recombinant IL-2 |
R and D Systems |
402 ML-020 |
|
| Peptide, recombinant protein |
Recombinant IL-4 |
R and D Systems |
404 ML-010 |
|
| Peptide, recombinant protein |
Recombinant IL-10 |
R and D Systems |
417 ML |
|
| Commercial assay, kit |
IFNγ ELISA MAX |
Biolegend |
430803 |
|
| Commercial assay, kit |
IL-4 ELISA MAX |
Biolegend |
431102 |
|
| Commercial assay, kit |
IL-10 ELISA MAX |
Biolegend |
431413 |
|
| Commercial assay, kit |
IL-17A ELISA MAX |
Biolegend |
432501 |
|
| Commercial assay, kit |
MOG35-55/CFA Emulsion PTX |
Hooke Laboratories |
EK-2110 |
|
| Chemical compound, drug |
STAT5i |
Stemcell |
73852 |
|
| Chemical compound, drug |
House dust mite |
Greer |
XPB8103A2.5 |
|
| Chemical compound, drug |
Ovalbumin |
Sigma-Aldrich |
A5503 |
|
| Chemical compound, drug |
Cockroach antigen |
Greer |
XPB46D3A4 |
|
| Chemical compound, drug |
Methacholine |
Sigma-Aldrich |
62-51-1 |
|
| Software, algorithm |
Prism |
Graphpad |
Versions 5 and 7, RRID:SCR_002798
|
|
| Software, algorithm |
FlowJo |
FlowJo |
v10.7, RRID:SCR_008520
|
|
| Other |
FACS-ARIA SORP |
BD |
|
|
| Other |
Attune NxT |
Thermo Fisher |
|
|
| Other |
Sytox Red |
Invitrogen |
S34859 |
|
| Other |
Fixable Viability Stain 510 |
BD |
564406 |
|
| Other |
MOG I-A(b) tetramer-BV421 |
NIH Tetramer Core |
GWYRSPFSRVVH |
|
| Other |
Control I-A(b) tetramer-BV421 |
NIH Tetramer Core |
PVSKMRMATPLLMQA |
|
| Other |
CellTrace Violet |
Invitrogen |
C34557 or C34571
|
|
| Other |
Europium-conjugated streptavidin |
Perkin-Elmer |
1244–360 |
|
| Other |
DELFIA Enhancement solution |
Perkin-Elmer |
4001–0010 |
|
| Other |
10% formalin |
Anachemia |
41916–700 |
|
| Other |
Hematoxylin |
Ricca |
3536–32 |
|
| Other |
Eosin |
EMD Harleco |
588X-75 |
|
| Other |
Solvent Blue 38 |
Sigma-Aldrich |
S3382 |
|
| Other |
Percoll |
GE |
17-0891-02 |
|
