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. 2021 Feb 15;2021:5619761. doi: 10.1155/2021/5619761

Figure 1.

Figure 1

Flavonoid profiles of the extracts of Perilla frutescens var. acuta (Odash.) Kudo leaves (PLE). (a) Comparative high-performance liquid chromatography ultraviolet (HPLC-UV) chromatograms of PLE ethanol extracts (top) and rosmarinic acid (RA, bottom) in the standard solution were obtained using an Agilent 1260 series liquid chromatography system. Chromatographic separation was carried out on a ZORBAX Eclipse Plus C18 column (4.6 × 150 mm, 5 μm), and the column temperature was maintained at 35°C. The mobile phase consisted of water containing 0.1% phosphoric acid (A) and acetonitrile (B). The composition of the mobile phase was 0–4 min, 0% (B); 10 min, 4% (B); 20 min, 10% (B); 30 min, 20% (B); 40 min, 40% (B); and 70 min, 100% (B). RA was detected at concentrations ranging from 38 to 40 min. PLE contained 4.6 mg/g of RA. (b) The chemical structure of RA isolated from PLE.