Figure 3.

miR-224-5p accelerates cell-cycle progression and inhibits apoptosis

(A–E) Representative results (A) and quantification of the cell-cycle distributions in H1299 and A549 cells stably transfected with pLenti/pLenti-miR-224 (B and C), and transiently transfected with NC/miR-224-5p inhibitor (D and E). Green, yellow, and blue represent the populations of cells in the G₀/G₁, S, and G₂/M phases, respectively. (F–I) Representative results (F and G) and quantification of the rate of apoptosis in H1299 and A549 cells, stably transfected with pLenti/pLenti-miR-224 (H), and transiently transfected with NC/miR-224-5p inhibitor (I). Cell-cycle distribution and rate of apoptosis were detected by flow cytometry. Error bars represent the mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.