Figure 7.

miR-224-5p drives tumor growth in vivo

(A and B) Representative images (A) and weights (B) of subcutaneous xenograft tumors from nude mice 8 weeks after injection with pLenti or pLenti-miR-224 A549 cells. (C) Tumor growth curves with volume were measured weekly after injection. (D and E) qRT-PCR analysis of miR-224-5p (D) and AR mRNA expression (E) in subcutaneous xenograft tumors. (F) Histopathology of metastases in the lungs of two groups of nude mice stained with hematoxylin and eosin (original magnification, ×100). (G) Representative images of tumor tissues stained with hematoxylin and eosin solution and representative images of immunohistochemistry staining for Ki67, E-cadherin, N-cadherin, and AR expression in tumor tissues (original magnification, ×100). Error bars represent the mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.