FIGURE 3.

Lack of effect of Azan-7 in S. epidermidis cultures. (A) S. epidermidis (10⁶ CFU/mL) were cultured in presence of Azan-7 (100 μM) or 0.05% DMSO (vehicle). Bacterial growth was evaluated 16 h later by measuring the optical density (OD) at 600 nm. Data are reported as mean ± SEM (n = 3). (B) S. epidermidis (10⁶ CFU/mL) were treated with Azan-7 (100 μM) or DMSO 0.05% for 16 h. rnaIII mRNA transcript levels were determined by qRT-PCR. Results are reported as log2 of relative gene expression. Data are reported as mean ± SEM (n = 3). (C) Comparison of AgrA protein aminoacidic sequence between S. epidermidis (first line) and MRSA (second line). AgrA proteins show homology of 87%. * = identity; . = semi-conservative amino acid; : = conservative amino acid; gap = non conservative amino acid (UniProt).