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. 2021 Feb 9;12:610859. doi: 10.3389/fmicb.2021.610859

TABLE 1.

Sequences and annealing temperatures of oligonucleotides used in the qRT-PCR experiments.

Genes Oligonucleotides Annealing temp (°C)
16S fw 5′-AAACTCAAAKGAATTGACGG-3′ 60°C
rv 5′-CTCACRRCACGAGCTGAC-3′
gyrB fw 5′-CAAATGATCACAGCATTTGGTACAG-3′ 60°C
rv 5′-CGGCATCAGTCATAATGACGAT-3′
rnaIII S. aureus fw 5′-TTCACTGTGTCGATAATCCA-3′ 60°C
rv 5′TGATTTCAATGGCACAAGAT-5′
rnaIII S. epidermidis fw 5′-ACTAAATCACCGATTGTAGAAATGATAT CT-3′ 62°C
rv 5′-ATTTGCTTAATCTAGTCGAGTGAATG TT A-3′
agrA fw 5′-GCACATACACGCTTACAATTGTTG-3′ 59°C
rv 5′-ACACTGAATTACTGCCACGTTTTAAT-3′
hla fw 5′ATGGATAGAAAAGCATCCAAACA-3′ 62°C
rv 5′-TTTCCAATTTGTTGAAGTCCAAT-3′
psmα fw 5′-TATCAAAAGCTTAATCGAACAATTC-3′ 60°C
rv 5′-CCCCTTCAAATAAGATGTTCATATC-3′
cap1A fw 5′-AGGCATGTCATGAGCAAAAACT-3′ 62°C
rv 5′-TGTCTTGTAGTAAGTGCGGCAT-3′
cap1C fw 5′-TTGCACATCCAGAGCGGAAT-3′ 62°C
rv 5′-TGCGCATCTGAACCGATGAA-3′