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. 2021 Feb 9;12:589518. doi: 10.3389/fpls.2021.589518

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Copyright © 2021 Soto, Prabhakar, Wang, Backe, Chapla, Bartetzko, Black, Azadi, Peña, Pfrengle, Moremen, Urbanowicz and Hahn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Linkage analysis of the reaction products of arabinan oligosaccharides incubated with GFP-AtFUT4. (A) Unreacted arabinobiose and arabinobiose incubated with GFP-AtFUT4. (B) Unreacted arabinotriose and arabinotriose incubated with GFP-AtFUT4. (C) Unreacted arabinotetraose and arabinotetraose incubated with GFP-AtFUT4. The presence of the 1,2-Araf peak in the spectra of the reaction products indicates that Fuc is attached to the terminal non-reducing Ara in all the oligosaccharides. As the 1,3,5-Araf and the 1,2,5-Araf peaks appear in the unreacted and reacted spectra we do not ascribe these to transfer in those linkages.