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. 2021 Feb 9;12:589518. doi: 10.3389/fpls.2021.589518

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Copyright © 2021 Soto, Prabhakar, Wang, Backe, Chapla, Bartetzko, Black, Azadi, Peña, Pfrengle, Moremen, Urbanowicz and Hahn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Imaging and staining of 12-day-old AtFUT4::GUS and AtFUT6::GUS seedlings. Multiple (n > 15) independent AtFUT4::GUS and AtFUT6::GUS plant lines are generated, grown, and observed for each construct. Visualization and qualitative analysis of GUS staining patterns of roots for two representative lines are shown. Staining patterns for lateral roots of increasing lengths are shown in (A,B,D,E), while close-ups of tap roots are shown in (C,F). (A,B) AtFUT4::GUS-1, (C) AtFUT4::GUS-2, (D,E) AtFUT6::GUS-1, and (F) AtFUT6::GUS-2.