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. 2021 Feb 9;12:570093. doi: 10.3389/fmicb.2021.570093

FIGURE 5.

FIGURE 5

Progesterone inhibits N. gonorrhoeae -induced NLRP3 inflammasome activation and IL-1β secretion in THP-1 macrophages. (A) PMA-differentiated THP-1 cells were pre-treated with N. gonorrhoeae overnight and then stimulated with P4. Four hours later supernatants were analyzed by ELISA for IL-1β release. (B) Cells were selected to detect the protein levels of NLRP3 and Caspase-1p20. (C,D) Semi quantification analysis of NLRP3 and caspase-1p20 western blot films using ImageJ software. (E,F) The mRNA levels of pro-IL-1β and NLRP3 on N. gonorrhoeae infected human differentiated THP-1 macrophages. (G,H) Cell supernatants from activated THP-1 cells were evaluated for active IL-1β by Western blot, semi quantification analysis of active IL-1β western blot films using ImageJ software. *p < 0.05, **p < 0.01. Data are presented as the mean ± SD, n = 5. GC, Neisseria gonorrhoeae; P4, progesterone.