TABLE 1.

Gene regions and respective primer pairsused in the study.

Gene region	Primers	Sequence5′-3′	Optimized PCR protocols	References
ITS	ITS1	TCCGTAGGTGAACCTGCGG	94°C: 5 min, (94°C: 30 s, 55°C: 50 s, 72°C: 1 min) × 34 cycles 72°C: 10 min	White et al., 1990
	ITS4	TCCTCCGCTTATTGATATGC
tub2	BT2a	GGTAACCAAATCGGTGCTGCTTTC	94°C: 5 min, (94°C: 30 s, 58°C: 50 s, 72°C: 1 min) × 34 cycles 72°C: 10 min	Glass and Donaldson, 1995
	Bt2b	ACCCTCAGTGTAGTGACCCTTGGC
tef1	EF1-728F	CATCGAGAAGTTCGAGAAGG	95°C: 5 min, (95°C: 30 s, 58°C: 30 s, 72°C: 1 min) × 34 cycles 72°C: 10 min	Carbone and Kohn, 1999
	EF1-986R	TACTTGAAGGAACCCTTACC
cal	CAL-228F	GAGTTCAAGGAGGCCTTCTCCC	95°C: 5 min, (95°C: 30 s, 55°C: 50 s, 72°C: 1 min) × 34 cycles 72°C: 10 min	Carbone and Kohn, 1999
	CAL-737R	CATCTTCTGGCCATCATGG