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. 2021 Jan 5;34(2):588–597. doi: 10.1007/s13577-020-00470-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Overexpression of MAFG-AS1 led to the upregulated SphK1. The effects of MAFG-AS1 and miR-125b-5p on the expression of SphK1, a direct target of miR-125b-5p, were analyzed by qPCR and western blot at mRNA (a) and protein (b) levels, respectively. RT-qPCR and Western blot were performed at 48 h post-transfection. All experiments were repeated 3 times and mean values were presented. *, p < 0.05