Fig. 1.

Overexpression of miR-27b-3p attenuated TGF-β1-mediated EMT in HK-2 cells. a HK-2 cells were treated with 5 ng/ml TGF-β1 for 48 h. RT-qPCR was used to measure the level of miR-27b-3p in HK-2 cells. b The level of miR-27b-3p in HK-2 cells transfected with miR-27b-3p agonist was detected using qRT-PCR. c HK-2 cells were transfected with miR-27b-3p agonist for 6 h, and then exposed to 5 ng/ml TGF-β1 for 48 h. Expressions of α-SMA, Collagen III, Fibronectin and Vimentin in HK-2 cells were detected with western blotting. Images are representative of three independent experiments. d–g The relative expressions of α-SMA, Collagen III, Fibronectin and Vimentin in HK-2 cells were quantified via normalization to GAPDH. **p < 0.01, compared with the control group. ^##p < 0.01, compared with the TGF-β1 group