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. 2021 Feb 9;9:634160. doi: 10.3389/fcell.2021.634160

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Copyright © 2021 Hu, Gao, Huang, Wang, Mo, Wang, Zhang, Xie, Li and Yao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flotillin-1 physically interacts with TRPV2. (A) Yeast two-hybrid results showing the interaction between TRPV2 N-terminus (V2-Nt) and flotillin-1. The bait and prey constructs were co-transformed into yeast strain AH109, and the transformed yeast diploids were grown on DDO for 2 days and QDO for 5 days, respectively. The 53-T and Lam-T were served as positive and negative controls, respectively. DDO, SD-Leu/-Trp medium; QDO, SD-Leu/-Trp/-His/-Ade medium; X, X-α-gal. (B) Immunoprecipitation (with anti-TRPV2, anti-Flotillin-1, or IgG as a control) and immunoblot analysis (with anti-TRPV2 or anti-Flotillin-1) of the mouse DRG neurons. (C) Interaction between flotillin-1 and TRPV2. HEK 293T cells were transfected with plasmids as indicated. Immunoprecipitation (IP, with anti-Flag) and immunoblot analysis (with anti-GFP and anti-Flag) of the interaction between TRPV2 and flotillin-1. (D) No interaction between flotillin-1 and TRPV1. Immunoprecipitation (with anti-Flag) and immunoblot analysis (with anti-GFP and anti-Flag) of HEK 293T cells transfected with plasmids encoding Flag-tagged TRPV1 and GFP-tagged flotillin-1. (E) No interaction between TRPV2 and flotillin-2. Immunoprecipitation (with anti-Flag) and immunoblot analysis (with anti-GFP and anti-Flag) of HEK 293T cells transfected with plasmids encoding Flag-tagged TRPV2 and GFP-tagged flotillin-2. Data are representative of three independent experiments at least.