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. 2021 Feb 9;9:634650. doi: 10.3389/fcell.2021.634650

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Copyright © 2021 Chen, Liang, Ji, Kou, Zhang, Shang, Shang, Song, Yang, Liu, Wang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Schematic illustration. (B) Relative mRNA expressions of osteogenic gene ALP, Runx-2, OCN, and OPN in BMSCs stimulated by different CM for 3 and 7 days. (C) Western blot results of Runx-2 and OCN expression in BMSCs on days 3 and 7. (D) Quantitation of the protein expression levels of Runx-2 and OCN. (E,F) ALP staining pictures and analysis of the BMSCs cultured in corresponding CM for 7 days. (G,H) ARS images and quantitative analysis of the BMSCs cultured with corresponding CM for 14 days. ##P < 0.01, compared with the NC group. ^∗P < 0.05, ^∗∗P < 0.01.