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. 2021 Feb 22;12:1213. doi: 10.1038/s41467-021-21447-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Representative images (from three independent experiments) of MDA-MB-231 cell density 96 h after the start of high-contrast bright-field imaging. Cells were treated with either control or KIF18A siRNAs in combination with DMSO or 500 nM UMK57. Scale bar is 100 microns. b Fold change in cell density after 96 h in MDA-MB-231 cells treated with the specified siRNAs and either 500 nM UMK57 or DMSO. n = 76 (control KD + DMSO), 52 (control KD + UMK57), 76 (KIF18A KD + DMSO), and 68 (KIF18A KD + UMK57) wells of cells from three independent experiments. Data were analyzed via one-way ANOVA with post hoc Tukey’s test for multiple comparisons. c, d Percent of total mitotic cells (c) and mitotic cells with multipolar spindles (d) in fixed populations after the indicated treatments. n = 3 independent experimental replicates per condition. Data were analyzed via one-way ANOVA with post hoc Tukey’s test for multiple comparisons. e, f Percent of live, siR-tubulin-labeled MDA-MB-231 cells that (e) split poles or (f) entered mitosis with more than two spindle poles after the indicated treatments. n = 126 (control KD + DMSO), 158 (control KD + UMK57), 172 (KIF18A KD + DMSO), and 152 (KIF18A KD + UMK57) mitotic cells from three independent experiments. Data were analyzed via a two-sided Chi-square test. All graphs show mean ± SD and individual data points. P values <0.05 are displayed.