Fig. 1. Identification of 133 conopeptide genes from the assembled genome of C. betulinus.
a Image of a representative C. betulinus. b The 17-mer distribution of sequenced reads. These sequencing data generated from the Illumina short-insert libraries were used for a genome survey analysis8. The x-axis represents the sequencing depth of each 17-mer, and the y-axis represents the percentage of each unique 17-mer. c Four representative types of exon distribution in the identified full-length conopeptide genes. Regions in purple, green, red, and black stand for the 1st, 2nd, 3rd, and 4th exons, respectively. d Eight deduced protein sequences of these genes in c. Sequences highlighted in black, yellow, and red are signal peptides, pro-/post-peptides and mature peptides of these precursors, respectively. e Phylogeny of identified conopeptide genes based on predicted signal peptide sequences. f Detailed distribution of exon length.