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. 2021 Feb 22;12:1220. doi: 10.1038/s41467-021-21443-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Side and top view of the Ub-R:Ube2N~Ub:Ube2V2 structure (Ub-R, Ub in red, R in blue, Ube2N~Ub, Ube2N in green, Ub in orange, and Ube2V2 in teal). Chains drawn as cartoon represent the asymmetric unit. b The canonical model of initiation of RING-anchored ubiquitin chain elongation. c Schematic cartoon, representing the canonical model of RING-anchored ubiquitin chain elongation shown in b. Symmetry mates are denoted by ′ next to the label. Ub ubiquitin, Ub-R ubiquitin-RING.