Figure 3.

G-CatchER⁺ response in hippocampal neurons

(A) 0.5 mM of 4-cmc was added to initiate a RyR-dependent release of Ca²⁺ from the ER in mouse primary hippocampal neurons.

(B) Corresponding bar graph of 4-cmc activated Ca²⁺ release (in ΔF/F₀) from the ER grouped by neuron regions. Error bars are ±SEM, ∗p = 0.05, one-way ANOVA, Tukey's multiple comparisons.

(C) Inhibition of SERCA with 50 μM of CPA initiated a release of Ca²⁺ from the ER.

(D) Corresponding bar graph of CPA inhibited Ca²⁺ release (in ΔF/F₀) from the ER grouped by neuron regions. Error bars are ±SEM, one-way ANOVA, Tukey's multiple comparisons.

(E) 100 μM of DHPG was added to initiate the release of Ca²⁺ from the ER via mGluR1/5 activation in hippocampal neurons.

(F) Corresponding bar graph of DHPG-induced Ca²⁺ release (in ΔF/F₀) from the ER grouped by neuron regions. Error bars are ±SEM, ∗p = 0.02, one-way ANOVA, Tukey's multiple comparisons.

See also Figure S4.