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Comparison of particle distribution of Mrp in free-standing ice and on continuous carbon support
Cryo-EM grids of the Mrp complex under different conditions as labeled.
(A) 0.2% DDM; 3 mg/mL Mrp.
(B) 0.15% LMNG; 2 mg/mL Mrp.
(C) 0.2% DDM +0.2% CHAPS; 1.5 mg/mL Mrp.
(D) 0.19% LMNG +0.2% CHAPS; 2 mg/mL Mrp.
(E) 0.2% DDM; 2.2 mg/mL of FLAG tag Mrp.
(F) Mrp in pMAL-C8.
(G) Intact Mrp particles in LMNG on continuous carbon support.
(H) Freeze-dried Mrp particles on carbon support.
