Figure 3.

TGFβRi treatment alters mitochondrial function and cell proliferation in VICs

(A) Assessment of mitochondrial function and cell proliferation following 48-h treatment with TGFβR inhibitors. Mitochondrial function was measured by ATP production, oxidative stress (ROS generation), and cellular mitochondrial respiration. Proliferation was evaluated by CyQUANT. Concentration-responsive decreases in production of ATP and ROS and proliferation were observed following TGFβRi treatment.

(B) Cellular mitochondrial respiration evaluation by Seahorse. Additional evaluation of mitochondrial respiration with LY2109761 following 48-h treatment demonstrated concentration-related decreases, which was also observed with other TGFβR inhibitors (data not shown).

Represented data are the average of three experiments ±SEM. Statistical comparisons employed one-way or two-way ANOVA followed by Dunnett's test. ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.005; ∗∗∗∗p ≤ 0.0001.