Figure 5.
TGFβRi treatment induces altered morphology and migration of VICs
(A) Morphological changes of VICs labeled with α-SMAgreen and vimentinred following 48-h treatment with 10 μM TGFβRis. Nuclei were labeled with DAPIblue. Middle panels show 2.5× enlargement of boxed area of α-SMA, vimentin, and DAPI merged. Bottom panels show boxed area with only vimentin and DAPI signal.
(B) VICs were assessed over a time course for changes in cell membrane impedance. Impedance will increase as cells become more adherent. Cellular impedance increased in DMSO-treated controls but decreased following treatment with LY2109761 and BMT-A. In contrast, there was minimal change in impedance following BMT-B treatment.
(C) VIC migration was quantitated over a 48 h time course using the scratch test. The percent cell density corrected from T0 baseline is presented. LY2109761 and BMT-A presented less cell migration compared with DMSO and BMT-B.
Represented data in (B and C) are the average of three experiments ± SEM. Statistical comparisons employed one-way or two-way ANOVA followed by Dunnett's test. ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.005; ∗∗∗∗p ≤ 0.0001. Scale bars, 100 μm.