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. 2021 Feb 22;12:1210. doi: 10.1038/s41467-021-21306-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Growth experiments of a wild type B. subtilis (black), GP3407 (ΔdarB; gray), wild type + pGP3306 (darB⁺, blue). b GP3419 (Δrel, bright green) and GP3419 (Δrel, darB⁺, dark green), and c wild type + pGP3437/3441/3601 (darB^A25G, darB^R132M, darB^A25G,R132M, pink) in MSSM minimal medium with 0.1 mM KCl (upper panel) or 5 mM KCl (lower panel). Growth was monitored in an Epoch 2 Microplate Spectrophotometer (BioTek Instruments) at 37 °C with linear shaking at 237 cpm (4 mm) for 12 h. The growth experiment was conducted three times and a representative curve is shown. Source data are provided as a Source data file.