Fig. 4.

Quantitative evaluation of the 2-NBDG and 2-NBDLG uptake into U2OS cells examined by a laser confocal microscope. a Changes in the mean fluorescence intensities of U2OS cells examined at 7 DIV before and after administration of KRB solution containing 200 μM of 2-NBDG or 2-NBDLG. Data are the means ± SD of total fluorescence intensity of individual ROIs. b Net increase in the fluorescence in (a) was expressed as percent increase in the fluorescence relative to that for 2-NBDG administration. Numbers in parenthesis show the number of ROIs that were assigned as much as possible (14–24) for each of 7 pre-determined areas of the cover slip. Similar results were obtained in three separate experiments