Table 4.

Insulin, HIF1α, and VEGF in BRIN-BD11 with and without actinomycin D, and mRNA insulin level in BRIN-BD11

Cultured time under hypoxia (min)	Pancreatic endocrine cell culture (BRIN-BD11)					Pancreatic endocrine cell culture (BRIN-BD11) with actinomycin D
	Insulin (ng/mL)	Ins1/β-actin	Ins2/β-actin	HIF1α (ng/mL) (median)	VEGF (ng/mL) (median)	Insulin (ng/mL) (median)	HIF1α (ng/mL) (median)	VEGF (pg/mL) (median)
0	5.58–6.99 (6.49)	1.00–1.31 (1.11)	1.00–1.15 (1.02)	0.48–3.10 (1.74)	1017.0–1250.0 (1209)	3.12–4.56 (4.35)	1.07–5.11 (3.20)	367.4–485.9 (439.2)
5	5.89–6.36 (6.23)	0.72–1.33 (0.97)	0.50–0.84 (0.52)	0.52–4.19 (2.32)	957.7–1190.0 (1014)	2.40–7.10 (3.12)	0.68–5.68 (3.08)	368.2–484.3 (428.1)
10	6.58–7.46 (7.36)	1.78–2.55 (1.92)	1.02–1.30 (1.27)	0.59–6.59 (1.92)	950.0–1159.0 (1131)	3.70–4.58 (4.33)	2.46–5.37 (2.63)	396.8–478.9 (432.5)
15	5.89–6.73 (6.47)	1.39–1.77 (1.55)	0.47–0.76 (0.75)	0.45–5.66 (2.92)	1247.0–1694.0 (1336)	3.97–5.43 (4.22)	0.83–8.54 (2.59)	391.1–552.0 (413.9)
30	4.86–5.25 (5.06)	0.89–1.59 (0.91)	0.31–1.59 (0.88)	2.97–7.33 (4.39)	1138.0–1684.0 (1401)	3.50–4.72 (4.22)	1.45–5.42 (2.74)	391.9–540.7 (438.4)
60	4.73–5.45 (5.39)	1.06–1.27 (1.12)	0.55–1.07 (0.92)	1.19–4.46 (2.47)	1062.0–1331.0 (1268)	3.69–4.72 (4.07)	0.65–7.63 (4.32)	376.7–501.4 (449.8)