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. 2021 Feb 9;11:621463. doi: 10.3389/fmicb.2020.621463

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Copyright © 2021 Borrego and Brun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Kinetics of growth in mammalian and insect cells. Vero cells (A) and HEK293 (B) were infected at a MOI of 0.01 while C6/36 mosquito cells (C) were infected with 0.005 pfu/cell. After 1 h of adsorption the inoculum was removed, cells were washed and fresh medium was added. Supernatants were collected at different times post infection (pi) and titrated on Vero cell monolayers Independent titrations were performed in duplicates in at least two independent experiments. Mean values ± SD are represented. Significance level was set to *P < 0.05 (multiple t-test using the Holm–Sidak method, with alpha = 5.000%). Data shown correspond to one representative experiment. (D) Plaque phenotypes of the indicated viruses on Vero cell monolayers.