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. 2021 Feb 9;9:616219. doi: 10.3389/fcell.2021.616219

TABLE 2.

Parameters of primer pairs for the genes.

Gene Primer sequence Product size GenBank accession
PPARα F: 5′-TAACGGAGTTCCAATCGC-3′ 222 bp NM 001001464
R: 5′-AACCCTTACAACCTTCACAA-3′
PPARγ F: 5′-CACTGCAGGAACAGAACAAAGAA-3′ 67 bp NM 001001460
R: 5′-TCCACAGAGCGAAACTGACATC-3′
PKB F: 5′-CTGATGATGCCAAGGAGATT-3′ 175 bp NM 205055.1
R: 5′-TGGTCAGGAGGAGTGATTGT-3′
AMPKα1 F: 5′-AAGGTTGGCAAGCATGAGTT-3′ 492 bp NM 001039603
R: 5′-TTCTGGGCCTGCATATAACC-3′
AMPKα2 F: 5′-AGCACGCCAACAGAGACTTCTT-3′ 399 bp NM 001039605
R: 5′-ATCATCAAAGGGCAAAGTGC-3′
AMPKβ1 F: 5′-CCAGGAGCCCTATGTCTGTAAG-3′ 113 bp NM 001039912
R: 5′-CAGGATCGCAAGAAATGCC-3′
AMPKβ2 F: 5′-GCACTGCCCATCCCTCTAA-3′ 125 bp NM 001044662
R: 5′-CGGCTGCCACGAAACAA-3′
AMPKγ F: 5′-AGAGGTCCCAAAGCCTGAGTT-3′ 118 bp NM 001034827
R: 5′-GAAGATGCCCAGAGCCACA-3′
PI3K F: 5′-CGGATGTTGCCTTACGGTTGT-3′ 162 bp NM 001004410.1
R: 5′-GTTCTTGTCCTTGAGCCACTGAT-3′
ACC F: 5′-CACTTCGAGGCGAAAAACTC-3′ 447 bp NM 205505
R: 5′-GGAGCAAATCCATGACCACT-3′
CPT1 F: 5′-CAATGAGGTACTCCCTGAAA-3′ 337 bp NM oo102898
R: 5′-CATTATTGGTCCACGCCCTC-3′
HMGCR F: 5′-AGCTGCAACCCTGAGGAAACT-3′ 1268 bp AB109635
R: 5′-AGCCATCACTGTAGCACACAC-3′
SREBP1 F: 5′-GAGGAAGGCCATCGAGTCA-3′ 392 bp AY 029224
R: 5′-GGAAGACAAAGGCACAGAGG-3′
LKB1 F: 5′-GGGGAGACAGAAGGGAACAGA-3′ 158 bp NM 001045833
R:5′-TGAGAGGGATGCTTGAATACGA-3′
β-actin F: 5′-TGCGTGACATCAAGGAGAAG-3′ 300 bp L08165
R: 5′-TGCCAGGGTACATTGTGGTA-3′
18sRNA F:5′-TAGATAACCTCGAGCCGATCGCA-3′ 312 bp AF 173612
R:5′-GACTTGCCCTCCAATGGATCC TC-3′