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. 2021 Feb 9;9:619596. doi: 10.3389/fcell.2021.619596

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Copyright © 2021 Kunz, Lehmann and Pohl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RPN-6.1 is required for proper AM pore and dendrite morphogenesis. (A) Maximum intensity projections of stacks of confocal images from time lapse recordings of left side views of embryo after control injection illustrating the movement of the sensilla pores (green) correlated with elongation of the AM dendrites (magenta). White arrowheads point to AM pores/dendrite tips. (B) Depictions as in A, however, rpn-6.1 RNAi embryos are shown. Injection of dsRNA leads to different levels of penetrance (from top to bottom). Yellow arrowheads highlight impaired AM pore and/or dendrite morphogenesis. See also Supplementary Video 8. (C) Quantification of the effects of rpn-6.1 RNAi on AM pore movement (left) and on AM dendrite morphogenesis (right) (n > 5, ±SD; two-way ANOVA P; *** ≥ 0.001; **** ≥ 0.0001).