FIGURE 6.
RPN-6.1 is required for the morphogenesis of organs with apical lumen. (A) Maximum intensity projections of stacks of confocal images from time lapse recordings of left side view of embryo after control injection; apically polarized surfaces (sensory organ pores, excretory pore, pharynx, intestine) are shown in green, and AM neurons and dendrites are shown in magenta. (B) Depictions as in A, however, for rpn-6.1 RNAi embryos. Yellow arrowheads mark discontinuities of pharynx or intestine, respectively. See also Supplementary Video 9. (C) Quantification of phenotypes observed in rpn-6.1 RNAi embryos (n = 29 for AM and n = 22 for all other phenotypes). None of the phenotypes has been observed in control injected embryos (n ≥ 20).
