FIGURE 7.

Apical constriction, sensory organ pore movement and the role of RPN-6.1. (A) Top: Maximum intensity projections of stacks of confocal images from time lapse recordings of a representative wt embryo imaged from anterior. The edge of the epidermis was tracked based on cell membranes and is highlighted by a dashed line. The AM pores are highlighted by white arrowheads. Bottom left: Magnified views of cell shape changes and identification of individual sensory organ socket cells. Anterior apical tips of cells are marked with arrowheads. Bottom right: Quantification of sensory organ pore (socket cell) movement (n = 5). (B) Top: Maximum intensity projections of stacks of confocal images from time lapse recordings of a representative wt embryo imaged from the left side. Bottom: Magnified single z-stacks from the boxed area above. Arrowheads mark the AM pore, individual neuronal and arcade cells are outlined to illustrate their shape change. (C) Representation as in panel (B), however, for a representative, moderately RPN-6.1 depleted embryo. See also Supplementary Video 12.