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. 2021 Feb 9;9:568738. doi: 10.3389/fcell.2021.568738

Figure 3.

Figure 3

Upregulation of miR-128-3p and downregulation of FOXO4 induced EMT in CRC cells. qRT-PCR of (A) miR-128-3p and (B) FOXO4 mRNA expression in HCT-116 cells transfected with miR-128-3p overexpression or interference vectors (or corresponding NC). (C) qRT-PCR of FOXO4 mRNA expression in HCT-116 cells transfected with FOXO4 overexpression or interference vectors (or corresponding NC). CCK-8 detection of the viability of HCT-116 cells after transfection with overexpression or interference vectors (or corresponding NC) of (D) miR-128-3p and (E) FOXO4. Wound healing assay (scale bar = 200 μm) after HCT-116 cells were transfected with overexpression or interference vectors (or corresponding NC) of (F) miR-128-3p and (G) FOXO4. Transwell invasion assay (scale bar = 100 μm) after HCT-116 cells were transfected with overexpression or interference vectors (or corresponding NC) of (H) miR-128-3p and (I) FOXO4. Data are shown as the mean ± SD (n = 3); *P < 0.05.