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. 2021 Feb 9;9:568738. doi: 10.3389/fcell.2021.568738

Figure 4.

Figure 4

miR-128-3p overexpression and FOXO4 interference regulated the expression of EMT markers and TGF-β/SMAD and JAK/STAT3 signaling. HCT-116 cells were transfected with overexpression or interference vectors (or corresponding NC) of miR-128-3p or FOXO4. (A) Western blot and quantification of EMT markers E-cadherin, ZO-1, vimentin, and N-cadherin in HCT-116 cells. Protein expression was normalized to that of GAPDH. (B) Western blot and quantification of TGF-β, SMAD2, and SMAD3 in HCT-116 cells. Protein expression was normalized to that of GAPDH. (C) Western blot and quantification of p-JAK2, JAK2, p-JAK3, JAK3, p-STAT3, and STAT3 in HCT-116 cells. Phosphorylated protein expression was normalized to that of total proteins. Data are shown as the mean ± SD (n = 3); *P < 0.05.