Skip to main content
. 2021 Feb 9;9:568738. doi: 10.3389/fcell.2021.568738

Figure 5.

Figure 5

FOXO4 inhibited miR-128-3p-induced EMT in CRC cells. HCT-116 cells were transfected with overexpression or interference vectors of miR-128-3p and/or FOXO4. qRT-PCR of (A) miR-128-3p expression and (B) FOXO4 mRNA expression in HCT-116 cells. (C) Western blot and quantification of FOXO4 protein expression in HCT-116 cells. Protein expression was normalized to that of GAPDH. (D) Western blot and quantification of EMT markers E-cadherin, ZO-1, vimentin, and N-cadherin in HCT-116 cells. Protein expression was normalized to that of GAPDH. (E) Wound healing assay, scale bar = 200 μm. Data are shown as the mean ± SD (n = 3); *P < 0.05.