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. 2021 Feb 9;11:523769. doi: 10.3389/fphys.2020.523769

Figure 3.

Figure 3

Dose dependent effects of LGZ (A,B), SN (E,F), PCM (I,J); and SN combined with LGZ (C,D) or PCM (G,H) on mechanical and heat hypersensitivities in mice with carrageenan induced inflammatory pain. N = 6–8 mice, data were presented as mean ± SEM. Black or Gray lines represent the average (mechanical/heat) thresholds before or 1 day after carrageenan injection, respectively. LGZ was applied at the dosages of 10, 20, and 80 mg/kg (A,B); SN was applied at the dosages of 10, 20, and 80 mg/kg (E,F); PCM was applied at the dosages 10, 30, and 100 mg/kg (I,J); SN (at 10 or 20 mg/kg) were applied 60 min before LGZ (at 10 or 20 mg/kg; C,D), or PCM (at 10 or 30 mg/kg; G,H) administrations. Mechanical/heat thresholds were measured for 240 min after completion of drug applications. Two Way ANOVA with repeated measures indicated overall significant differences (P < 0.05) between the groups (A,B,E,F,G,H,J). *P < 0.05, **P < 0.01, ***P < 0.001, post-drug thresholds were compared with pre-drug baselines at 0 min, using Dunn's (A,C,E,G,I) or Dunnett's (B,D,F,H,J) multiple comparisons test following ANOVA; #P < 0.05, ##P < 0.01, ###P < 0.001, post-drug effects were compared with controls (vehicle treatments) at each time points, using Bonferroni's multiple comparisons test following ANOVA.