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. 2021 Feb 9;10:584024. doi: 10.3389/fonc.2020.584024

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Copyright © 2021 Jakobsen, Traynor, Stæhr, Duijf, Nielsen, Terp, Pedersen, Guldberg, Ditzel and Gjerstorff

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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VCX2 protein expression is limited to testis among healthy adult tissues. (A) The specificity of a VCX2 specific antibody used for immunohistochemical analysis toward VCX2 was confirmed by western blotting using lysates from HEK293T cells with ectopic expression of VCX2 and VCX. The antibody only recognized VCX2 and not VCX or other endogenously expressed proteins. (B) VCX2 protein expression was investigated in a panel of 23 different normal adult tissues. Expression was only observed in testis among these tissues, where nuclear staining was seen in germ cells at all stages of spermatogenesis. Only a subset of tissues is shown. Magnification: × 40.