Figure 2.

VCX2 expression in cancer and subcellular localization. (A) VCX2 protein expression was observed in 4/19 melanoma cell lines (FM45, FM79, A375, and Sk-Mel-37b) and 1/11 breast cancer cell lines (T-47-D) (see Supplementary Table 1 for a complete list). (B) The antibody was confirmed to recognize a protein similar in size to VCX2 in melanoma cell lysate from cells positive in immunohistochemical staining (FM45) and did not react with a lysate from cells negative in immunohistochemical staining (FM3). (C) Among 261 cancer specimens derived from 19 different histological origins, VCX2 protein expression was only observed in melanoma, where expression was seen in 4/31 cancer specimens (see Table 1 for a complete list). Only a subset of cancer cell lines and cancer specimens are shown. (D) Investigation of VCX2 subcellular localization in A375 cells, using immunocytochemistry, showed the presence of the protein only in the nucleus, with a localization pattern similar to chromatin. Magnification: × 40 (A, C) × 60 (D).