Table 2.
HCMV ppp65 epitope category distribution in HCMV positive, HLA-A*02:01 positive subjects.
| Test Subjects' CD8+ T Cells Specific for Epitopes | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| ID 1 | ID 2 | ID 3 | ID 4 | ID 5 | ID 6 | ID 7 | ID 8 | ID 9 | ID 10 | ||
| Cryptic Epitopes | |||||||||||
| Number | 11 | 23 | 32 | 24 | 6 | 2 | 14 | 3 | 8 | 21 | |
| Cum. SFU | 12.10 | 18.67 | 67.15 | 92.50 | 3.04 | 0.39 | 58.40 | 11.53 | 21.57 | 56.12 | |
| % of total SFU | 1.02% | 4.41% | 3.02% | 13.54% | 0.73% | 0.24% | 4.03% | 1.04% | 2.89% | 5.09% | |
| Subdominant Epitopes | |||||||||||
| Number | 14 | 8 | 5 | 17 | 6 | 2 | 6 | 0 | 1 | 16 | |
| Cum. SFU | 45 | 35 | 63 | 230 | 14 | 21 | 194 | 0 | 7 | 132 | |
| % of total SFU | 4% | 8% | 3% | 34% | 3% | 13% | 13% | 0% | 1% | 12% | |
| Dominant Epitopes | |||||||||||
| Number | 15 | 4 | 3 | 4 | 9 | 0 | 3 | 1 | 0 | 5 | |
| Cum. SFU | 281 | 72 | 147 | 193 | 263 | 0 | 178 | 73 | 0 | 221 | |
| % of total SFU | 24% | 17% | 7% | 28% | 63% | 0% | 12% | 7% | 0% | 20% | |
| Super Dominant Epitopes | |||||||||||
| Number | 3 | 1 | 4 | 2 | 1 | 1 | 3 | 2 | 2 | 3 | |
| Cum. SFU | 847 | 298 | 1948 | 168 | 139 | 139 | 1019 | 1027 | 717 | 694 | |
| % of total SFU | 71% | 70% | 88% | 25% | 33% | 86% | 70% | 92% | 96% | 63% | |
| Total Epitopes Recognized | 43 | 36 | 44 | 47 | 22 | 5 | 26 | 6 | 11 | 45 | |
| Cumulative Spec. SFU | 1185 | 424 | 2226 | 683 | 418 | 161 | 1450 | 1111 | 746 | 1103 | |
The number of cryptic, subdominant, dominant and super-dominant epitopes, as defined in the text, are shown for the individual test subjects, along with the sum of epitopes in each category (Total Epitopes Recognized) for each PBMC donor. The absolute number of CD8+ T cells targeting peptides in each category (Cum. SFU) is also shown. From the number of all pp65-specific CD8+ T cells detected in each subject (Cumulative Spec. SFU) the percentage of CD8+ T cells targeting peptides in each of the four response categories has been calculated (% of total SFU). The SFU counts shown are after subtracting the mean + 3 SD specificity cut off value. Because SFU counts for the cryptic category frequently were at the cut-off value, or barely exceeded it, they shown with two decimal places.