FIGURE 7.

An overview of the unfolding pathway of SOD1 as observed from the DTIMS ^DTCCS_N2 data. The native structure of SOD1 is a holo-dimer comprised of two monomers, each containing one Zn²⁺ and one Cu²⁺ ion. After addition of acid to the solution, the SOD1 holo-dimer elongated and dissociated into holo-monomers. The holo-monomers were further denatured from their native state by loss of a metal ligand, leading to the formation of a single-metal monomer. Further loss of the additional metal ion led to the formation of the apo-monomer, which extended to the largest ^DTCCS_N2 values observed for all protein structures.