FIGURE 2.

Brachyury (T) transforms the adipose‐derived mesenchymal stem cell transcriptome. A, Heat map was generated using RNA sequencing data from cell lysates at five time points; 0‐ (pre), 24‐, 48‐, 72‐, and 96‐hours after T‐transfection. Each time point consisted of biological triplicates (n = 3). Quantile‐adjusted conditional maximum likelihood method (qCML) was used for the initial bioinformatics analysis. B, Gene Ontology analysis of differentially expressed genes (upregulated at 72 hours vs 0 hour, based on P < .05 and log2‐fold change >1 cutoff) identified biological processes with enriched representation. T‐induced transcriptome prioritization included antioxidant/response to stimuli (green); angiogenesis/circulatory development (red); immunomodulatory/anti‐inflammatory response (yellow); cell, tissue, and organ development/differentiation (blue). These biological functions are all considered to be of potential benefit in cardiac repair