FIGURE 4.

Angiogenicity and vasculogenic secretome. A, Green fluorescent protein (GFP)‐tagged human umbilical vein endothelial cells were treated with: vascular endothelial growth factor (+VEGF) as positive control, LY83583 as negative control (+LY83583), adipose‐derived mesenchymal stem cell conditioned media (+AMSC CM), or eCP conditioned media (+eCP CM). Conditioned media was extracted from the same starting volume (one confluent T75 flask, 10 mL volume) and 200 μL was added to 2 mL of media per well. Among four conditions, the +eCP CM group displayed superior vasculogenesis quantified by vessel area, junction, and length. Scale bar = 500 μm; *P < .05 with one‐way ANOVA followed by a post hoc Bonferroni test (biological replicates [n] ≥ 4 per group). B, eCP CM, compared to AMSC CM, contained a higher concentration of proteins. **P < .01 with Student's t test (n = 4 per group). C, Multiple angiogenesis‐related proteins were identified in eCP CM. After normalizing background signal and protein concentration of original blots (left panels) and their quantification, top 24 proteins from the 55‐protein array are depicted (n = 1 with technical duplicates; right panel). ANOVA, analysis of variance