Figure 1.

A system for analyzing transcription-replication coordination

(A) Experimental design schematic and FACS analysis of propidium iodide (PI) and BrdU to monitor S-phase progression, with quantification of cells number in each box (bottom left, G0/G1 phase; top, S phase; bottom right, G2 phase; n = 3).

(B) Representative genomic view of BrdU-seq (in blue) and Chr-RNA-seq (in red) on the long arm of chromosome 7, with a 95-Mb view at all time points.

(C) Single-gene analysis of BrdU incorporation levels as percentage of the input at each time point over the indicated genes; n = 3.

(D) Nascent transcription levels as relative ratio to G1/S compared to the fold changes measured by Chr-RNA-seq; n = 3 for RT-PCR, n = 2 for Chr-RNA-seq. Data represent mean ± SEM. Student’s t test; ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001.