Fig. 1.

Effect of α-mangostin (α-mg) on cell viability, survival rate, and cell proliferation. α-mg (0, 3, 6, 9 μM) was administered to B16F10 cells for 72 h. Cell viability was analyzed by MTT assay (A). After 72 h treatment, medium and cells were collected, and the cell pellet was finally put together. The cell survival rate was examined by trypan blue staining (B). CT indicates cells cultured in the normal medium; since the α-mg was dissolved in DMSO, we also added DMSO in 0 μM α-mg groups. Besides, α-mg (3, 6, 9 μM) was administered to B16F10 cells and the number of cells was measured daily, in a state where α-mg was kept treating for 6 days from day 0 to day 5 and the effect on cell proliferation was investigated (C). Mean ± SD; **p < 0.01 vs. CT (A); *p < 0.05, **p < 0.01 vs. 0 μM (B, C). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)