Fig. 2.

Effect of α-mg on melanin synthesis. B16F10 cells were cultured with α-mg (0, 3, 6, 9 μM) administered for 72 h. Cells were collected by trypsinization and centrifuged to obtain pellets (A). The pellets were dissolved in 1 M NaOH, and the relative amount of melanin in each experimental group was determined by measuring the absorbance at 475 nm. α-mg dose-dependently decreased the melanin content in cells (B). α-MSH (10 μM) was used as a positive control for melanin synthesis, which was administered at the same time as α-mg. α-mg also decreased melanin content with the presence of α-MSH (C). Mean ± SD; **p < 0.01 vs. 0 μM (B); *p < 0.05 vs. 0 μM (-α-MSH), p < 0.01 vs. 0 μM (+α-MSH) (C).