Fig. 3.

α-mg suppresses intercellular tyrosinase activity through down-regulating tyrosinase and MITF gene expression. B16F10 cells were cultured with α-mg (0, 3, 6, 9 μM) administered for 72 h. The same quantity of cells lysate was mixed with l-DOPA, the melanin synthesized in 40 min, measured by plate-reader at 475 nm wavelength, was recognized as tyrosinase activity. α-mg decreased the tyrosinase activity over 6 μM (A). the protein level was tested by Western blot. All these three genes were decreased by α-mg treatment (B). Mean ± SD; **p < 0.01, ***p < 0.005 vs. 0 μM.