Fig. 4.

The effect of α-mg is mediated through the ERK and GSK3β pathways.

B16F10 cells were cultured with α-mg (0, 3, 6, 9 μM) administered for 72 h. α-mg treatment increased pERK and pGSK3β without regulating ERK and GSK3β gene expression (A). U0126 was used to treat cells, and it increased melanin in cells. Melanin in U0126 cotreatment with α-mg group lower than U0126 group and higher than α-mg group (B). BIO was used to treat cells, and it also increased melanin in cells. Melanin in BIO cotreatment with α-mg group had no significant difference with BIO (C). Mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.005 vs. non-treatment group (-/-); #p < 0.05, ###p < 0.005 vs. co-treatment group (+/+); n. s. non-significance.