Fig. 5.

Effect of α-mg on cell proliferation and melanin synthesis in NHEMs. NHEMs were cultured with α-mg (0, 0.25, 0.5, 0.75, 1 μM) for 72 h, and cell viability was examined by MTT assay (A). CT means experiment group cultured in medium alone; 0 μM α-mg in medium containing 0.07% EtOH as a solvent. The cells were collected by trypsin and centrifuged to obtain pellets (B). The pellet was dissolved in 1 M NaOH and the amount of melanin in each experimental group was determined by measuring the absorbance at 475 nm (C). Mean ± SD; **p < 0.01 vs. CT (A); *p < 0.05, **p < 0.01 vs. 0 μM (C).