Fig. 2.
Determination of kinetic parameters of uracil excision by MtuUng: [A] Background subtracted continuous graph showing linear relationship between product formation and substrate concentration in the presence of MtuUng. The enzyme concentration of 0.3 x 10−9 M was used with the substrate concentrations ranging from 40 x 10−9 to 640 x 10−9 M, n = 3. [B] Graph of the reaction velocities, calculated as the slopes of the data from [A], as a function of the oligomer concentration depicting the Michaelis-Menten trend. [C] The Lineweaver-Burk graph from data in [B] with inverse range of substrate concentration from 40x10−9-640x10−9 M to determine Km and Vmax, n = 3, R2 = 0.97.