Fig. 3.
Dose-dependent inhibition analysis of MtuUng by uracil: [A] Normalised graph illustrating inverse relation of inhibitor (uracil) concentration (1 x 10−3 to 25 x 10−3 M) towards fluorescence production. Uracil was dissolved in DMSO (dimethyl-sulfoxide) and treated against 0.3 x 10−9 M enzyme in the presence of 30 x 10−9 M of substrate (n = 3). [B] The linear time course of graph A is then used to analyse relative product conversion with respect to log concentration of the inhibitor to determine dose-dependent response (IC50) of uracil on MtuUng (n = 3).