Fig. 4.
Inhibition kinetics of MtuUng by 5-chloro-uracil: [A] Kinetics of MtuUng inhibition by chloro-uracil as double reciprocal plots in the presence of different inhibitor concentrations. The inhibitors were dissolved in DMSO and used with 0.3 x 10−9 M of the enzyme in the presence of 30 x 10−9 M of the oligomer. The double-reciprocal graph interprets the type of inhibition by chloro-uracil towards MtuUng (n = 3). [B] Secondary plot of slopes from Lineweaver-Burk graph as a function of inhibitor concentration to determine Ki as x-intercept (n = 3).