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. 2021 Feb 15;26:100954. doi: 10.1016/j.bbrep.2021.100954

Table 1.

List of compounds screened with Dose-dependent inhibition analysis: All the compounds were treated with 0.3 x 10−9 M of MtuUng followed by addition of 30 x 10−9 M of the fluorescence -labelled oligomer. The reaction volume was taken to be 100 μl buffered by 50 mM Tris-Cl (pH 8), 1 mM Na2EDTA and 37.59 nM BSA (n = 3).

S. No. Inhibitor Structure Docking Score IC50 in mM (mean ± SEM)
1 Uracil Image 1 −10.268 2.05 ± 0.3
2 4-Thio-Uracil Image 2 −4.806 3.57 ± 0.35
3 5- Iodo-Uracil Image 3 −9.103 4.92 ± 0.51
4 5-Chloro-Uracil Image 4 −8.805 8.71 ± 0.70
5 5-Fluoro-Uracil Image 5 −8.963 50.18 ± 3.85
6 5-Nitro-Uracil Image 6 −5.054 10.77 ± 0.32
7 5-Amino-Uracil Image 7 −9.636 7.18 ± 0.97
8 5-Bromo-Uracil Image 8 −8.733 13.59 ± 0.73
9 Barbituric acid Image 9 −12.03 7.29 ± 0.32