Figure 1.

Transient mouse MCAO/reperfusion model and the lipidomic profiling workflow.A, a schema of the time course to generate the MCAO model, tissue analysis, and lipid function studies. B, a schema illustrates the workflow to obtain lipid metabolites and their functions. C, TTC staining of coronal brain sections at 2 mm thickness to show ischemic (white-colored on the right hemisphere) and the contralateral side (red-colored tissue on the left hemisphere). D, Laser Doppler flowmetry measurements of blood flow of the middle cerebral artery before and after MCAO. E, the infarct volume measured from the TTC stained brain coronal sections. F, neurological deficits scores were measured from MCAO and Sham group of mice for up to 28 days reperfusion. The forepaw pulling strength test (G) and rotor rod performance test (H) from the Sham and MCAO groups. Data represent the mean ± SD. Error bars indicate SD. n = 8, ∗ indicates p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 by one-way ANOVA with LSD post hoc analysis to identify significant groups when compared between MCAO with the Sham group.